A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis

S. K. Mohanty; S. K. Ravula; K. L. Engisch; A. B. Frazier

doi:10.1109/SENSOR.2003.1216950

A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis

S. K. Mohanty, S. K. Ravula, K. L. Engisch, A. B. Frazier

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (µ-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for µ-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy.

Original language	English
Title of host publication	TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)
Publisher	IEEE
Pages	1055-1058
Number of pages	4
ISBN (Print)	0-7803-7731-1
DOIs	https://doi.org/10.1109/SENSOR.2003.1216950
State	Published - 2003
Externally published	Yes
Event	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States Duration: Jun 8 2003 → Jun 12 2003

Conference

Conference	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
Country/Territory	United States
City	Boston
Period	6/8/03 → 6/12/03

ASJC Scopus Subject Areas

Electrical and Electronic Engineering

Keywords

Bovine
Calcium
Cells (biology)
Dielectrophoresis
Electrochemical impedance spectroscopy
Frequency
Impedance measurement
Micromachining
Micromechanical devices
Red blood cells

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Neurosciences
Physiological Processes

Access to Document

10.1109/SENSOR.2003.1216950License: Unspecified

Cite this

Mohanty, S. K., Ravula, S. K., Engisch, K. L., & Frazier, A. B. (2003). A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis. In TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664) (pp. 1055-1058). Article 1216950 IEEE. https://doi.org/10.1109/SENSOR.2003.1216950

A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis. / Mohanty, S. K.; Ravula, S. K.; Engisch, K. L. et al.
TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664). IEEE, 2003. p. 1055-1058 1216950.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Mohanty, SK, Ravula, SK, Engisch, KL & Frazier, AB 2003, A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis. in TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)., 1216950, IEEE, pp. 1055-1058, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 6/8/03. https://doi.org/10.1109/SENSOR.2003.1216950

Mohanty SK, Ravula SK, Engisch KL, Frazier AB. A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis. In TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664). IEEE. 2003. p. 1055-1058. 1216950 doi: 10.1109/SENSOR.2003.1216950

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title = "A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis",

abstract = " Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (µ-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for µ-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy.",

keywords = "Bovine, Calcium, Cells (biology), Dielectrophoresis, Electrochemical impedance spectroscopy, Frequency, Impedance measurement, Micromachining, Micromechanical devices, Red blood cells",

author = "Mohanty, {S. K.} and Ravula, {S. K.} and Engisch, {K. L.} and Frazier, {A. B.}",

note = "Publisher Copyright: {\textcopyright} 2003 IEEE.; 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers ; Conference date: 08-06-2003 Through 12-06-2003",

year = "2003",

doi = "10.1109/SENSOR.2003.1216950",

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T1 - A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis

AU - Mohanty, S. K.

AU - Ravula, S. K.

AU - Engisch, K. L.

AU - Frazier, A. B.

PY - 2003

Y1 - 2003

N2 - Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (µ-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for µ-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy.

AB - Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (µ-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for µ-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy.

KW - Bovine

KW - Calcium

KW - Cells (biology)

KW - Dielectrophoresis

KW - Electrochemical impedance spectroscopy

KW - Frequency

KW - Impedance measurement

KW - Micromachining

KW - Micromechanical devices

KW - Red blood cells

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BT - TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)

PB - IEEE

T2 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers

Y2 - 8 June 2003 through 12 June 2003

ER -

A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis

Abstract

Conference

ASJC Scopus Subject Areas

Keywords

Disciplines

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