A Single Cell Multi-Analysis System for Electrophysiological Studies

A. Han; E. Moss; R. D. Rabbitt; K. L. Engisch; A. B. Frazier

doi:10.1109/SENSOR.2003.1215563

A Single Cell Multi-Analysis System for Electrophysiological Studies

A. Han, E. Moss, R. D. Rabbitt, K. L. Engisch, A. B. Frazier

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A micro analysis system for multi-purpose electrophysiological studies of single cells has been designed, fabricated, and characterized. The micro electrophysiological analysis system (µ-EPAS) contains multiple extracellular opposing electrode pairs that interface with a single cell inside an analysis cavity. These electrodes can be used for general single electrode extracellular stimulation/recording studies, dual electrode applications such as impedance spectroscopy, and for applications requiring a greater number of electrodes such as impedance tomography. Additionally, the analysis system contains a sealing/clamping mechanism and a backside extracellular reference electrode for patch clamping studies. The analysis cavities were designed in an array format for parallel analyses. Bovine chromaffin cells were used as a target to demonstrate and characterize the system. Using the integrated glass sealing ring, giga-seals were obtained on a population of chromaffin cells. The micro system generated maximum giga-seal resistances of 1.2GΩ with non-sealed average cavity-to-backside resistances of 0.5MΩ. Furthermore, the cellular analysis system was demonstrated for electrical impedance spectroscopy on chromaffin cells over a range from 40.0Hz to 1.0MHz.

Original language	American English
Title of host publication	TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)
Publisher	IEEE
Pages	674-677
Number of pages	4
ISBN (Print)	0-7803-7731-1
DOIs	https://doi.org/10.1109/SENSOR.2003.1215563
State	Published - 2003
Externally published	Yes
Event	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States Duration: Jun 8 2003 → Jun 12 2003

Conference

Conference	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
Country/Territory	United States
City	Boston
Period	6/8/03 → 6/12/03

ASJC Scopus Subject Areas

Electrical and Electronic Engineering

Keywords

Biochemical analysis
Biomembranes
Bovine
Cells (biology)
Chemical analysis
Clamps
Electrochemical impedance spectroscopy
Electrodes
Extracellular
Performance analysis

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Neurosciences
Physiological Processes

Access to Document

10.1109/SENSOR.2003.1215563License: Unspecified

Cite this

Han, A., Moss, E., Rabbitt, R. D., Engisch, K. L., & Frazier, A. B. (2003). A Single Cell Multi-Analysis System for Electrophysiological Studies. In TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664) (pp. 674-677). Article 1215563 IEEE. https://doi.org/10.1109/SENSOR.2003.1215563

A Single Cell Multi-Analysis System for Electrophysiological Studies. / Han, A.; Moss, E.; Rabbitt, R. D. et al.
TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664). IEEE, 2003. p. 674-677 1215563.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Han, A, Moss, E, Rabbitt, RD, Engisch, KL & Frazier, AB 2003, A Single Cell Multi-Analysis System for Electrophysiological Studies. in TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)., 1215563, IEEE, pp. 674-677, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 6/8/03. https://doi.org/10.1109/SENSOR.2003.1215563

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title = "A Single Cell Multi-Analysis System for Electrophysiological Studies",

abstract = " A micro analysis system for multi-purpose electrophysiological studies of single cells has been designed, fabricated, and characterized. The micro electrophysiological analysis system (µ-EPAS) contains multiple extracellular opposing electrode pairs that interface with a single cell inside an analysis cavity. These electrodes can be used for general single electrode extracellular stimulation/recording studies, dual electrode applications such as impedance spectroscopy, and for applications requiring a greater number of electrodes such as impedance tomography. Additionally, the analysis system contains a sealing/clamping mechanism and a backside extracellular reference electrode for patch clamping studies. The analysis cavities were designed in an array format for parallel analyses. Bovine chromaffin cells were used as a target to demonstrate and characterize the system. Using the integrated glass sealing ring, giga-seals were obtained on a population of chromaffin cells. The micro system generated maximum giga-seal resistances of 1.2GΩ with non-sealed average cavity-to-backside resistances of 0.5MΩ. Furthermore, the cellular analysis system was demonstrated for electrical impedance spectroscopy on chromaffin cells over a range from 40.0Hz to 1.0MHz.",

keywords = "Biochemical analysis, Biomembranes, Bovine, Cells (biology), Chemical analysis, Clamps, Electrochemical impedance spectroscopy, Electrodes, Extracellular, Performance analysis",

author = "A. Han and E. Moss and Rabbitt, {R. D.} and Engisch, {K. L.} and Frazier, {A. B.}",

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year = "2003",

doi = "10.1109/SENSOR.2003.1215563",

language = "American English",

isbn = "0-7803-7731-1",

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T1 - A Single Cell Multi-Analysis System for Electrophysiological Studies

AU - Han, A.

AU - Moss, E.

AU - Rabbitt, R. D.

AU - Engisch, K. L.

AU - Frazier, A. B.

PY - 2003

Y1 - 2003

N2 - A micro analysis system for multi-purpose electrophysiological studies of single cells has been designed, fabricated, and characterized. The micro electrophysiological analysis system (µ-EPAS) contains multiple extracellular opposing electrode pairs that interface with a single cell inside an analysis cavity. These electrodes can be used for general single electrode extracellular stimulation/recording studies, dual electrode applications such as impedance spectroscopy, and for applications requiring a greater number of electrodes such as impedance tomography. Additionally, the analysis system contains a sealing/clamping mechanism and a backside extracellular reference electrode for patch clamping studies. The analysis cavities were designed in an array format for parallel analyses. Bovine chromaffin cells were used as a target to demonstrate and characterize the system. Using the integrated glass sealing ring, giga-seals were obtained on a population of chromaffin cells. The micro system generated maximum giga-seal resistances of 1.2GΩ with non-sealed average cavity-to-backside resistances of 0.5MΩ. Furthermore, the cellular analysis system was demonstrated for electrical impedance spectroscopy on chromaffin cells over a range from 40.0Hz to 1.0MHz.

AB - A micro analysis system for multi-purpose electrophysiological studies of single cells has been designed, fabricated, and characterized. The micro electrophysiological analysis system (µ-EPAS) contains multiple extracellular opposing electrode pairs that interface with a single cell inside an analysis cavity. These electrodes can be used for general single electrode extracellular stimulation/recording studies, dual electrode applications such as impedance spectroscopy, and for applications requiring a greater number of electrodes such as impedance tomography. Additionally, the analysis system contains a sealing/clamping mechanism and a backside extracellular reference electrode for patch clamping studies. The analysis cavities were designed in an array format for parallel analyses. Bovine chromaffin cells were used as a target to demonstrate and characterize the system. Using the integrated glass sealing ring, giga-seals were obtained on a population of chromaffin cells. The micro system generated maximum giga-seal resistances of 1.2GΩ with non-sealed average cavity-to-backside resistances of 0.5MΩ. Furthermore, the cellular analysis system was demonstrated for electrical impedance spectroscopy on chromaffin cells over a range from 40.0Hz to 1.0MHz.

KW - Biochemical analysis

KW - Biomembranes

KW - Bovine

KW - Cells (biology)

KW - Chemical analysis

KW - Clamps

KW - Electrochemical impedance spectroscopy

KW - Electrodes

KW - Extracellular

KW - Performance analysis

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DO - 10.1109/SENSOR.2003.1215563

M3 - Conference contribution

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BT - TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)

PB - IEEE

T2 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers

Y2 - 8 June 2003 through 12 June 2003

ER -

A Single Cell Multi-Analysis System for Electrophysiological Studies

Abstract

Conference

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

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