Development of Chemosensitivity in Neurons from the Nucleus Tractus Solitarii (NTS) of Neonatal Rats

Susan C. Conrad; Nicole L. Nichols; Nick A. Ritucci; Jay B. Dean; Robert W. Putnam

doi:10.1016/j.resp.2008.11.005

Development of Chemosensitivity in Neurons from the Nucleus Tractus Solitarii (NTS) of Neonatal Rats

Susan C. Conrad, Nicole L. Nichols, Nick A. Ritucci, Jay B. Dean, Robert W. Putnam

Neuroscience, Cell Biology, and Physiology (NCBP)

Research output: Contribution to journal › Article › peer-review

Abstract

We studied the development of chemosensitivity during the neonatal period in rat nucleus tractus solitarii (NTS) neurons. We determined the percentage of neurons activated by hypercapnia (15% CO ₂ ) and assessed the magnitude of the response by calculating the chemosensitivity index (CI). There were no differences in the percentage of neurons that were inhibited (9%) or activated (44.8%) by hypercapnia or in the magnitude of the activated response (CI 164 ± 4.9%) in NTS neurons from neonatal rats of all ages. To assess the degree of intrinsic chemosensitivity in these neurons we used chemical synaptic block medium and the gap junction blocker carbenoxolone. Chemical synaptic block medium slightly decreased basal firing rate but did not affect the percentage of NTS neurons that responded to hypercapnia at any neonatal age. However, in neonates aged

Original language	American English
Journal	Respiratory Physiology Neurobiology
Volume	166
DOIs	https://doi.org/10.1016/j.resp.2008.11.005
State	Published - Mar 31 2009

Keywords

Hypercapnia
Intrinsic chemosensitivity
Gap junction
Ventilatory control

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Medical Sciences
Medicine and Health Sciences
Neurosciences
Physiological Processes

Access to Document

10.1016/j.resp.2008.11.005License: CC BY

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2683148/

Cite this

@article{efce4b1fe5bc44e098f0f7e44932e60b,

title = "Development of Chemosensitivity in Neurons from the Nucleus Tractus Solitarii (NTS) of Neonatal Rats",

abstract = " We studied the development of chemosensitivity during the neonatal period in rat nucleus tractus solitarii (NTS) neurons. We determined the percentage of neurons activated by hypercapnia (15% CO 2 ) and assessed the magnitude of the response by calculating the chemosensitivity index (CI). There were no differences in the percentage of neurons that were inhibited (9%) or activated (44.8%) by hypercapnia or in the magnitude of the activated response (CI 164 ± 4.9%) in NTS neurons from neonatal rats of all ages. To assess the degree of intrinsic chemosensitivity in these neurons we used chemical synaptic block medium and the gap junction blocker carbenoxolone. Chemical synaptic block medium slightly decreased basal firing rate but did not affect the percentage of NTS neurons that responded to hypercapnia at any neonatal age. However, in neonates aged",

keywords = "Hypercapnia, Intrinsic chemosensitivity, Gap junction, Ventilatory control",

author = "Conrad, {Susan C.} and Nichols, {Nicole L.} and Ritucci, {Nick A.} and Dean, {Jay B.} and Putnam, {Robert W.}",

year = "2009",

month = mar,

day = "31",

doi = "10.1016/j.resp.2008.11.005",

language = "American English",

volume = "166",

journal = "Respiratory Physiology Neurobiology",

}

TY - JOUR

T1 - Development of Chemosensitivity in Neurons from the Nucleus Tractus Solitarii (NTS) of Neonatal Rats

AU - Conrad, Susan C.

AU - Nichols, Nicole L.

AU - Ritucci, Nick A.

AU - Dean, Jay B.

AU - Putnam, Robert W.

PY - 2009/3/31

Y1 - 2009/3/31

N2 - We studied the development of chemosensitivity during the neonatal period in rat nucleus tractus solitarii (NTS) neurons. We determined the percentage of neurons activated by hypercapnia (15% CO 2 ) and assessed the magnitude of the response by calculating the chemosensitivity index (CI). There were no differences in the percentage of neurons that were inhibited (9%) or activated (44.8%) by hypercapnia or in the magnitude of the activated response (CI 164 ± 4.9%) in NTS neurons from neonatal rats of all ages. To assess the degree of intrinsic chemosensitivity in these neurons we used chemical synaptic block medium and the gap junction blocker carbenoxolone. Chemical synaptic block medium slightly decreased basal firing rate but did not affect the percentage of NTS neurons that responded to hypercapnia at any neonatal age. However, in neonates aged

AB - We studied the development of chemosensitivity during the neonatal period in rat nucleus tractus solitarii (NTS) neurons. We determined the percentage of neurons activated by hypercapnia (15% CO 2 ) and assessed the magnitude of the response by calculating the chemosensitivity index (CI). There were no differences in the percentage of neurons that were inhibited (9%) or activated (44.8%) by hypercapnia or in the magnitude of the activated response (CI 164 ± 4.9%) in NTS neurons from neonatal rats of all ages. To assess the degree of intrinsic chemosensitivity in these neurons we used chemical synaptic block medium and the gap junction blocker carbenoxolone. Chemical synaptic block medium slightly decreased basal firing rate but did not affect the percentage of NTS neurons that responded to hypercapnia at any neonatal age. However, in neonates aged

KW - Hypercapnia

KW - Intrinsic chemosensitivity

KW - Gap junction

KW - Ventilatory control

UR - https://corescholar.libraries.wright.edu/ncbp/708

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2683148/

U2 - 10.1016/j.resp.2008.11.005

DO - 10.1016/j.resp.2008.11.005

M3 - Article

C2 - 19056522

VL - 166

JO - Respiratory Physiology Neurobiology

JF - Respiratory Physiology Neurobiology

ER -

Development of Chemosensitivity in Neurons from the Nucleus Tractus Solitarii (NTS) of Neonatal Rats

Abstract

Keywords

Disciplines

Access to Document

Other files and links

Cite this