Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN)

Robert W. Putnam; Lynn K. Hartzler; Susan C. Conrad; Nick A. Ritucci; Jay B. Dean

doi:10.1096/fasebj.20.4.A785-d

Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN)

Robert W. Putnam, Lynn K. Hartzler, Susan C. Conrad, Nick A. Ritucci, Jay B. Dean

Research output: Contribution to conference › Abstract › peer-review

Abstract

We studied the CO ₂ -sensitivity of neurons from LC, NTS and RTN. Firing rate and intracellular pH (pHi) responses to hypercapnic acidosis (HA) were studied in neurons from neonatal rat brainstem slices (300 µm) using whole cell patch and fluorescence imaging techniques. In response to HA, >90% of LC, 46% of NTS and 42% of RTN neurons increased their firing rate. The magnitude of the HA-induced increase in firing rate, calculated as the chemosensitivity index (CI), was 137±5% (n=17), 153±6% (52), and 248±30% (18) for LC, NTS and RTN neurons, respectively. Upon exposure to synaptic block medium (SNB) (11.4 mM Mg ²⁺ ; 0.2 mM Ca ²⁺ ), the spontaneous firing rate of LC neurons increased from 0.9±0.1 to 2.2±0.5 Hz (10) (p2-sensitive neurons are intrinsically chemosensitive. Since pHi responses to HA are similar in neurons from these areas, the differences in their intrinsic chemosensitivity must reside in differences in their ion channels.

[Supported by NIH grants RO1 HL56683 and F32 HL80877.]

Original language	American English
Pages	A785-A786
DOIs	https://doi.org/10.1096/fasebj.20.4.A785-d
State	Published - Mar 6 2006
Event	2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference - Duration: Mar 6 2006 → Mar 6 2006

Conference

Conference	2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference
Period	3/6/06 → 3/6/06

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Neurosciences
Physiological Processes

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10.1096/fasebj.20.4.A785-d

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Putnam, R. W., Hartzler, L. K., Conrad, S. C., Ritucci, N. A., & Dean, J. B. (2006). Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN). A785-A786. Abstract from 2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference. https://doi.org/10.1096/fasebj.20.4.A785-d

Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN). / Putnam, Robert W.; Hartzler, Lynn K.; Conrad, Susan C. et al.
2006. A785-A786 Abstract from 2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference.

Research output: Contribution to conference › Abstract › peer-review

Putnam, RW, Hartzler, LK, Conrad, SC, Ritucci, NA & Dean, JB 2006, 'Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN)', 2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference, 3/6/06 - 3/6/06 pp. A785-A786. https://doi.org/10.1096/fasebj.20.4.A785-d

Putnam RW, Hartzler LK, Conrad SC, Ritucci NA, Dean JB. Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN). 2006. Abstract from 2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference. doi: 10.1096/fasebj.20.4.A785-d

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title = "Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN)",

abstract = " We studied the CO 2 -sensitivity of neurons from LC, NTS and RTN. Firing rate and intracellular pH (pHi) responses to hypercapnic acidosis (HA) were studied in neurons from neonatal rat brainstem slices (300 µm) using whole cell patch and fluorescence imaging techniques. In response to HA, >90% of LC, 46% of NTS and 42% of RTN neurons increased their firing rate. The magnitude of the HA-induced increase in firing rate, calculated as the chemosensitivity index (CI), was 137±5% (n=17), 153±6% (52), and 248±30% (18) for LC, NTS and RTN neurons, respectively. Upon exposure to synaptic block medium (SNB) (11.4 mM Mg 2+ ; 0.2 mM Ca 2+ ), the spontaneous firing rate of LC neurons increased from 0.9±0.1 to 2.2±0.5 Hz (10) (p2-sensitive neurons are intrinsically chemosensitive. Since pHi responses to HA are similar in neurons from these areas, the differences in their intrinsic chemosensitivity must reside in differences in their ion channels. [Supported by NIH grants RO1 HL56683 and F32 HL80877.]",

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doi = "10.1096/fasebj.20.4.A785-d",

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AU - Hartzler, Lynn K.

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AU - Ritucci, Nick A.

AU - Dean, Jay B.

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N2 - We studied the CO 2 -sensitivity of neurons from LC, NTS and RTN. Firing rate and intracellular pH (pHi) responses to hypercapnic acidosis (HA) were studied in neurons from neonatal rat brainstem slices (300 µm) using whole cell patch and fluorescence imaging techniques. In response to HA, >90% of LC, 46% of NTS and 42% of RTN neurons increased their firing rate. The magnitude of the HA-induced increase in firing rate, calculated as the chemosensitivity index (CI), was 137±5% (n=17), 153±6% (52), and 248±30% (18) for LC, NTS and RTN neurons, respectively. Upon exposure to synaptic block medium (SNB) (11.4 mM Mg 2+ ; 0.2 mM Ca 2+ ), the spontaneous firing rate of LC neurons increased from 0.9±0.1 to 2.2±0.5 Hz (10) (p2-sensitive neurons are intrinsically chemosensitive. Since pHi responses to HA are similar in neurons from these areas, the differences in their intrinsic chemosensitivity must reside in differences in their ion channels. [Supported by NIH grants RO1 HL56683 and F32 HL80877.]

AB - We studied the CO 2 -sensitivity of neurons from LC, NTS and RTN. Firing rate and intracellular pH (pHi) responses to hypercapnic acidosis (HA) were studied in neurons from neonatal rat brainstem slices (300 µm) using whole cell patch and fluorescence imaging techniques. In response to HA, >90% of LC, 46% of NTS and 42% of RTN neurons increased their firing rate. The magnitude of the HA-induced increase in firing rate, calculated as the chemosensitivity index (CI), was 137±5% (n=17), 153±6% (52), and 248±30% (18) for LC, NTS and RTN neurons, respectively. Upon exposure to synaptic block medium (SNB) (11.4 mM Mg 2+ ; 0.2 mM Ca 2+ ), the spontaneous firing rate of LC neurons increased from 0.9±0.1 to 2.2±0.5 Hz (10) (p2-sensitive neurons are intrinsically chemosensitive. Since pHi responses to HA are similar in neurons from these areas, the differences in their intrinsic chemosensitivity must reside in differences in their ion channels. [Supported by NIH grants RO1 HL56683 and F32 HL80877.]

UR - https://corescholar.libraries.wright.edu/ncbp/771

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DO - 10.1096/fasebj.20.4.A785-d

M3 - Abstract

SP - A785-A786

T2 - 2006 Federation of American Societies for Experimental Biology (FASEB) Science Research Conference

Y2 - 6 March 2006 through 6 March 2006

ER -

Differential Intrinsic Chemosensitivity of Brainstem Neurons from the Locus Coeruleus (LC), the Nucleus Tractus Solitarius (NTS) and the Retrotrapezoid Nucleus (RTN)

Abstract

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