Effect of Sciatic Nerve Transection or TTX Application on Enzyme Activity in Rat Spinal Cord

Patrick A. Carr; Valerie K. Haftel; Francisco J. Alvarez; Timothy C. Cope; Robert E.W. Fyffe

doi:10.1097/00001756-199801260-00033

Effect of Sciatic Nerve Transection or TTX Application on Enzyme Activity in Rat Spinal Cord

Patrick A. Carr, Valerie K. Haftel, Francisco J. Alvarez, Timothy C. Cope, Robert E.W. Fyffe

Neuroscience, Cell Biology, and Physiology (NCBP)

Research output: Contribution to journal › Article › peer-review

Abstract

To clarify the differential effects on spinal circuitry caused by physical vs functional disconnection from the periphery, we compared changes produced by 3-, 7- or 14-day unilateral sciatic axotomy or tetrodotoxin (TTX) nerve blockade on the abundance or activity of NADPH diaphorase (NDP), cytochrome oxidase (CO) and acid phosphatase (AP) in the spinal cord. Following axotomy, AP and NDP were decreased in the dorsal horn and increased in large cells in the dorsolateral motor nuclei while CO was decreased in ventral horn neuropil. TTX induced a decrease of CO in the ventral horn and NDP in the dorsal horn. This suggests that physical vs functional disconnection causes modulation of distinct intracellular pathways in sensory afferents, dorsal horn neurons and motoneurons.

Original language	American English
Pages (from-to)	357-361
Journal	Neuroreport
Volume	9
Issue number	2
DOIs	https://doi.org/10.1097/00001756-199801260-00033
State	Published - Jan 26 1998

Keywords

Acid phosphatase
Cytochrome oxidase
Dorsal horn
Motoneurons
Nerve injury
Nitric oxide

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Neurosciences
Physiological Processes

Access to Document

10.1097/00001756-199801260-00033

Cite this

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title = "Effect of Sciatic Nerve Transection or TTX Application on Enzyme Activity in Rat Spinal Cord",

abstract = " To clarify the differential effects on spinal circuitry caused by physical vs functional disconnection from the periphery, we compared changes produced by 3-, 7- or 14-day unilateral sciatic axotomy or tetrodotoxin (TTX) nerve blockade on the abundance or activity of NADPH diaphorase (NDP), cytochrome oxidase (CO) and acid phosphatase (AP) in the spinal cord. Following axotomy, AP and NDP were decreased in the dorsal horn and increased in large cells in the dorsolateral motor nuclei while CO was decreased in ventral horn neuropil. TTX induced a decrease of CO in the ventral horn and NDP in the dorsal horn. This suggests that physical vs functional disconnection causes modulation of distinct intracellular pathways in sensory afferents, dorsal horn neurons and motoneurons.",

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author = "Carr, {Patrick A.} and Haftel, {Valerie K.} and Alvarez, {Francisco J.} and Cope, {Timothy C.} and Fyffe, {Robert E.W.}",

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doi = "10.1097/00001756-199801260-00033",

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AU - Carr, Patrick A.

AU - Haftel, Valerie K.

AU - Alvarez, Francisco J.

AU - Cope, Timothy C.

AU - Fyffe, Robert E.W.

PY - 1998/1/26

Y1 - 1998/1/26

N2 - To clarify the differential effects on spinal circuitry caused by physical vs functional disconnection from the periphery, we compared changes produced by 3-, 7- or 14-day unilateral sciatic axotomy or tetrodotoxin (TTX) nerve blockade on the abundance or activity of NADPH diaphorase (NDP), cytochrome oxidase (CO) and acid phosphatase (AP) in the spinal cord. Following axotomy, AP and NDP were decreased in the dorsal horn and increased in large cells in the dorsolateral motor nuclei while CO was decreased in ventral horn neuropil. TTX induced a decrease of CO in the ventral horn and NDP in the dorsal horn. This suggests that physical vs functional disconnection causes modulation of distinct intracellular pathways in sensory afferents, dorsal horn neurons and motoneurons.

AB - To clarify the differential effects on spinal circuitry caused by physical vs functional disconnection from the periphery, we compared changes produced by 3-, 7- or 14-day unilateral sciatic axotomy or tetrodotoxin (TTX) nerve blockade on the abundance or activity of NADPH diaphorase (NDP), cytochrome oxidase (CO) and acid phosphatase (AP) in the spinal cord. Following axotomy, AP and NDP were decreased in the dorsal horn and increased in large cells in the dorsolateral motor nuclei while CO was decreased in ventral horn neuropil. TTX induced a decrease of CO in the ventral horn and NDP in the dorsal horn. This suggests that physical vs functional disconnection causes modulation of distinct intracellular pathways in sensory afferents, dorsal horn neurons and motoneurons.

KW - Acid phosphatase

KW - Cytochrome oxidase

KW - Dorsal horn

KW - Motoneurons

KW - Nerve injury

KW - Nitric oxide

UR - https://corescholar.libraries.wright.edu/ncbp/237

U2 - 10.1097/00001756-199801260-00033

DO - 10.1097/00001756-199801260-00033

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EP - 361

JO - Neuroreport

JF - Neuroreport

IS - 2

ER -

Effect of Sciatic Nerve Transection or TTX Application on Enzyme Activity in Rat Spinal Cord

Abstract

Keywords

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