Abstract
The aim of this study was to determine the effects of temperature upon pH, protein charge and acid-base-relevant ion exchange in air-breathing ectotherms. Plasma and skeletal muscles in cane toads (Bufo marinus) and bullfrogs (Rana catesbeiana) were examined at 30, 20 and 10 °C. In addition, skeletal muscle ion concentrations were examined in black racer snakes (Coluber constrictor) at 30 and 10 °C. Cooling the amphibians produced a reduction in most of the plasma ion concentrations (Na + , K + , Ca 2+ , Cl - , SO 4 2- ) and in protein concentration because of increased hydration. Between 30 and 10 °C, total plasma osmolality fell by 14 % in the toads and by 5 % in the frogs. Plasma protein charge, calculated using the principle of electroneutrality, was unaffected by temperature, except possibly for the toads at 10 °C. The in vivo skeletal muscle ΔpHi/ΔT ratio, where pHi is intracellular pH and T is temperature, between 30 and 20 °C averaged −0.014 °C -1 in the toads and −0.019 °C -1 in the frogs. Between 20 and 10 °C, there was no change in pHi in the toads and a −0.005 °C -1 change in the frogs. The in vitro skeletal muscle ΔpHi/ΔT averaged −0.011 °C -1 in both toads and frogs. In all three species, skeletal muscle inulin space declined with cooling. Intracellular ion concentrations were calculated by subtracting extracellular fluid ion concentrations from whole-muscle ion concentrations. In general, temperature had a large effect upon intracellular ion concentrations (Na + , K + , Cl - ) and intracellular CO 2 levels. The relevance of the changes in intracellular ion concentration to skeletal muscle acid-base status and protein charge and the possible mechanisms producing the adjustments in intracellular ion concentration are discussed. It is concluded that ion-exchange mechanisms make an important contribution to adjusting pH with changes in temperature.
Original language | English |
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Pages (from-to) | 2065-2074 |
Number of pages | 10 |
Journal | Journal of Experimental Biology |
Volume | 203 |
Issue number | 13 |
State | Published - Jul 2000 |
Externally published | Yes |
ASJC Scopus Subject Areas
- Ecology, Evolution, Behavior and Systematics
- Physiology
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology
- Insect Science
Keywords
- Acid-base physiology
- Amphibian
- Blood
- Bufo marinus
- Coluber constrictor
- Electrolyte
- PH
- Protein charge
- Rana catesbeiana
- Reptile
- Skeletal muscle
- Temperature
Disciplines
- Biology
- Life Sciences
- Medical Sciences
- Medicine and Health Sciences
- Systems Biology