Abstract
<p> <h4> OBJECTIVE: </h4></p><p> To investigate whether the PD-L expression in the liver cell lines transinfected with HBV (HepG2.2.15 cells) can be up-regulated after cytokines stimulating. <h4> METHODS: </h4></p><p> To apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-alpha and IFN-gamma (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating. <h4> RESULTS: </h4></p><p> Whether or not transinfected with HBV, IFN-alpha and IFN-gamma both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-alpha, IFN-gamma all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-gamma can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV. <h4> CONCLUSION: </h4></p><p> IFN-alpha, IFN-gamma both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.</p>
Original language | American English |
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Journal | Chinese Journal of Experimental and Clinical Virology |
Volume | 22 |
State | Published - Oct 1 2008 |
Keywords
- Cytokines/genetics
- Cytokines/metabolism
- Gene Expression
- Hepatitis B virus
- Hepatitis B/metabolism
- Hepatitis B/pathology
- Hepatocytes/Metabolism
- Hepatocytes/Virology
- Humans
- Liver/Pathology
Disciplines
- Chemicals and Drugs
- Digestive System Diseases
- Diseases
- Medicine and Health Sciences