Identification of γ-Aminobutyric Acid-Like Immunoreactive Neurons in the Rat Cuneate Nucleus

Vickie R. Roettger; John C. Pearson; Melvyn D. Goldfinger

doi:10.1016/0304-3940(89)90137-7

Identification of γ-Aminobutyric Acid-Like Immunoreactive Neurons in the Rat Cuneate Nucleus

Vickie R. Roettger, John C. Pearson, Melvyn D. Goldfinger

Neuroscience, Cell Biology, and Physiology (NCBP)

Research output: Contribution to journal › Article › peer-review

Abstract

Neurons in the cuneate nucleus of the rat were examined for γ-aminobutyric acid-like immunoreactivity (GABA-LI) using antiserum raised against GABA-glutaraldehyde-keyhole limpet hemocyanin. GABA-LI neurons were analyzed for size, shape, and distribution and compared to Nissl-stained neurons. GABA-LI cell bodies were located at all rostral-caudal levels and were distributed randomly throughout the nucleus except at the level of the obex, where they were limited to the peripheral region of the cuneate nucleus. GABA-LI cell bodies had a significantly smaller mean cross-sectional area than the total cuneate neuronal population and comprised 21.5% of the total neuronal population as assessed with Nissl-staining. These results are consistent with the hypothesis that GABA is involved in processing somatosensory information in the rat dorsal column nuclei.

Original language	American English
Journal	Neuroscience Letters
Volume	97
DOIs	https://doi.org/10.1016/0304-3940(89)90137-7
State	Published - Feb 13 1989

Keywords

Nucleus cuneatus
Immunohistochemistry
γ-Aminobutyric acid
Rat

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Medical Sciences
Medicine and Health Sciences
Neurosciences
Physiological Processes

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10.1016/0304-3940(89)90137-7License: CC BY

Cite this

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title = "Identification of γ-Aminobutyric Acid-Like Immunoreactive Neurons in the Rat Cuneate Nucleus",

abstract = " Neurons in the cuneate nucleus of the rat were examined for γ-aminobutyric acid-like immunoreactivity (GABA-LI) using antiserum raised against GABA-glutaraldehyde-keyhole limpet hemocyanin. GABA-LI neurons were analyzed for size, shape, and distribution and compared to Nissl-stained neurons. GABA-LI cell bodies were located at all rostral-caudal levels and were distributed randomly throughout the nucleus except at the level of the obex, where they were limited to the peripheral region of the cuneate nucleus. GABA-LI cell bodies had a significantly smaller mean cross-sectional area than the total cuneate neuronal population and comprised 21.5% of the total neuronal population as assessed with Nissl-staining. These results are consistent with the hypothesis that GABA is involved in processing somatosensory information in the rat dorsal column nuclei.",

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doi = "10.1016/0304-3940(89)90137-7",

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AU - Roettger, Vickie R.

AU - Pearson, John C.

AU - Goldfinger, Melvyn D.

PY - 1989/2/13

Y1 - 1989/2/13

N2 - Neurons in the cuneate nucleus of the rat were examined for γ-aminobutyric acid-like immunoreactivity (GABA-LI) using antiserum raised against GABA-glutaraldehyde-keyhole limpet hemocyanin. GABA-LI neurons were analyzed for size, shape, and distribution and compared to Nissl-stained neurons. GABA-LI cell bodies were located at all rostral-caudal levels and were distributed randomly throughout the nucleus except at the level of the obex, where they were limited to the peripheral region of the cuneate nucleus. GABA-LI cell bodies had a significantly smaller mean cross-sectional area than the total cuneate neuronal population and comprised 21.5% of the total neuronal population as assessed with Nissl-staining. These results are consistent with the hypothesis that GABA is involved in processing somatosensory information in the rat dorsal column nuclei.

AB - Neurons in the cuneate nucleus of the rat were examined for γ-aminobutyric acid-like immunoreactivity (GABA-LI) using antiserum raised against GABA-glutaraldehyde-keyhole limpet hemocyanin. GABA-LI neurons were analyzed for size, shape, and distribution and compared to Nissl-stained neurons. GABA-LI cell bodies were located at all rostral-caudal levels and were distributed randomly throughout the nucleus except at the level of the obex, where they were limited to the peripheral region of the cuneate nucleus. GABA-LI cell bodies had a significantly smaller mean cross-sectional area than the total cuneate neuronal population and comprised 21.5% of the total neuronal population as assessed with Nissl-staining. These results are consistent with the hypothesis that GABA is involved in processing somatosensory information in the rat dorsal column nuclei.

KW - Nucleus cuneatus

KW - Immunohistochemistry

KW - γ-Aminobutyric acid

KW - Rat

UR - https://corescholar.libraries.wright.edu/ncbp/697

U2 - 10.1016/0304-3940(89)90137-7

DO - 10.1016/0304-3940(89)90137-7

M3 - Article

C2 - 2919008

VL - 97

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

Identification of γ-Aminobutyric Acid-Like Immunoreactive Neurons in the Rat Cuneate Nucleus

Abstract

Keywords

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