Interleukin-2 is present in human blood vessels and released in biologically active form by heparanase

John D. Miller; Suzanne E. Clabaugh; Deandra R. Smith; R. Brian Stevens; Lucile E. Wrenshall

doi:10.1038/icb.2011.45

Interleukin-2 is present in human blood vessels and released in biologically active form by heparanase

John D. Miller, Suzanne E. Clabaugh, Deandra R. Smith, R. Brian Stevens, Lucile E. Wrenshall

Research output: Contribution to journal › Article › peer-review

Abstract

Interleukin-2 (IL-2) is a multifaceted cytokine with immunostimulatory and immunosuppressive properties. Our laboratory recently demonstrated that the availability of IL-2 is regulated, in part, by association with perlecan, a heparan sulfate proteoglycan. Given the abundance of perlecan in blood vessels, we asked whether IL-2 is present in vessel walls. Our results indicate that IL-2 is associated with endothelial and smooth muscle cells within the human arterial wall. This IL-2 is released by heparanase, and promotes the proliferation of an IL-2-dependent cell line. Given the presence of IL-2 in human arteries, we asked whether the large vessels of IL-2-deficient mice were normal. The aortas of IL-2-deficient mice exhibited a loss of smooth muscle cells, suggesting that IL-2 may contribute to their survival. In their entirety, these results suggest a here-to-fore unrecognized role of IL-2 in vascular biology, and have significant implications for both the immune and cardiovascular systems.

Original language	English
Pages (from-to)	159-167
Number of pages	9
Journal	Immunology and Cell Biology
Volume	90
Issue number	2
DOIs	https://doi.org/10.1038/icb.2011.45
State	Published - Feb 2012
Externally published	Yes

ASJC Scopus Subject Areas

Immunology and Allergy
Immunology
Cell Biology

Keywords

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Animals
Aortic Aneurysm / metabolism
Arteries / cytology
Arteries / metabolism
Cells, Cultured
Endothelial Cells / metabolism
Endothelium, Vascular / metabolism*
Extracellular Matrix Proteins / metabolism
Glucuronidase / metabolism*
Heparan Sulfate Proteoglycans / metabolism
Humans
Interleukin-2 / metabolism*
Mice
Mice, Knockout
Muscle, Smooth, Vascular / metabolism*
Receptors, Interleukin-2 / metabolism
Extracellular Matrix Proteins
Heparan Sulfate Proteoglycans
Interleukin-2
Receptors, Interleukin-2
perlecan
heparanase
Glucuronidase

Disciplines

Immunology and Infectious Disease
Medical Immunology
Cell Biology

Access to Document

10.1038/icb.2011.45

Cite this

@article{f4722b44bfef4c76ab7ddc0aaa45e0da,

title = "Interleukin-2 is present in human blood vessels and released in biologically active form by heparanase",

abstract = "Interleukin-2 (IL-2) is a multifaceted cytokine with immunostimulatory and immunosuppressive properties. Our laboratory recently demonstrated that the availability of IL-2 is regulated, in part, by association with perlecan, a heparan sulfate proteoglycan. Given the abundance of perlecan in blood vessels, we asked whether IL-2 is present in vessel walls. Our results indicate that IL-2 is associated with endothelial and smooth muscle cells within the human arterial wall. This IL-2 is released by heparanase, and promotes the proliferation of an IL-2-dependent cell line. Given the presence of IL-2 in human arteries, we asked whether the large vessels of IL-2-deficient mice were normal. The aortas of IL-2-deficient mice exhibited a loss of smooth muscle cells, suggesting that IL-2 may contribute to their survival. In their entirety, these results suggest a here-to-fore unrecognized role of IL-2 in vascular biology, and have significant implications for both the immune and cardiovascular systems.",

keywords = "Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Animals, Aortic Aneurysm / metabolism, Arteries / cytology, Arteries / metabolism, Cells, Cultured, Endothelial Cells / metabolism, Endothelium, Vascular / metabolism*, Extracellular Matrix Proteins / metabolism, Glucuronidase / metabolism*, Heparan Sulfate Proteoglycans / metabolism, Humans, Interleukin-2 / metabolism*, Mice, Mice, Knockout, Muscle, Smooth, Vascular / metabolism*, Receptors, Interleukin-2 / metabolism, Extracellular Matrix Proteins, Heparan Sulfate Proteoglycans, Interleukin-2, Receptors, Interleukin-2, perlecan, heparanase, Glucuronidase",

author = "Miller, {John D.} and Clabaugh, {Suzanne E.} and Smith, {Deandra R.} and Stevens, {R. Brian} and Wrenshall, {Lucile E.}",

year = "2012",

month = feb,

doi = "10.1038/icb.2011.45",

language = "English",

volume = "90",

pages = "159--167",

journal = "Immunology and Cell Biology",

issn = "0818-9641",

number = "2",

}

TY - JOUR

T1 - Interleukin-2 is present in human blood vessels and released in biologically active form by heparanase

AU - Miller, John D.

AU - Clabaugh, Suzanne E.

AU - Smith, Deandra R.

AU - Stevens, R. Brian

AU - Wrenshall, Lucile E.

PY - 2012/2

Y1 - 2012/2

N2 - Interleukin-2 (IL-2) is a multifaceted cytokine with immunostimulatory and immunosuppressive properties. Our laboratory recently demonstrated that the availability of IL-2 is regulated, in part, by association with perlecan, a heparan sulfate proteoglycan. Given the abundance of perlecan in blood vessels, we asked whether IL-2 is present in vessel walls. Our results indicate that IL-2 is associated with endothelial and smooth muscle cells within the human arterial wall. This IL-2 is released by heparanase, and promotes the proliferation of an IL-2-dependent cell line. Given the presence of IL-2 in human arteries, we asked whether the large vessels of IL-2-deficient mice were normal. The aortas of IL-2-deficient mice exhibited a loss of smooth muscle cells, suggesting that IL-2 may contribute to their survival. In their entirety, these results suggest a here-to-fore unrecognized role of IL-2 in vascular biology, and have significant implications for both the immune and cardiovascular systems.

AB - Interleukin-2 (IL-2) is a multifaceted cytokine with immunostimulatory and immunosuppressive properties. Our laboratory recently demonstrated that the availability of IL-2 is regulated, in part, by association with perlecan, a heparan sulfate proteoglycan. Given the abundance of perlecan in blood vessels, we asked whether IL-2 is present in vessel walls. Our results indicate that IL-2 is associated with endothelial and smooth muscle cells within the human arterial wall. This IL-2 is released by heparanase, and promotes the proliferation of an IL-2-dependent cell line. Given the presence of IL-2 in human arteries, we asked whether the large vessels of IL-2-deficient mice were normal. The aortas of IL-2-deficient mice exhibited a loss of smooth muscle cells, suggesting that IL-2 may contribute to their survival. In their entirety, these results suggest a here-to-fore unrecognized role of IL-2 in vascular biology, and have significant implications for both the immune and cardiovascular systems.

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Animals

KW - Aortic Aneurysm / metabolism

KW - Arteries / cytology

KW - Arteries / metabolism

KW - Cells, Cultured

KW - Endothelial Cells / metabolism

KW - Endothelium, Vascular / metabolism

KW - Extracellular Matrix Proteins / metabolism

KW - Glucuronidase / metabolism

KW - Heparan Sulfate Proteoglycans / metabolism

KW - Humans

KW - Interleukin-2 / metabolism

KW - Mice

KW - Mice, Knockout

KW - Muscle, Smooth, Vascular / metabolism

KW - Receptors, Interleukin-2 / metabolism

KW - Extracellular Matrix Proteins

KW - Heparan Sulfate Proteoglycans

KW - Interleukin-2

KW - Receptors, Interleukin-2

KW - perlecan

KW - heparanase

KW - Glucuronidase

UR - http://www.scopus.com/inward/record.url?scp=84857033243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857033243&partnerID=8YFLogxK

U2 - 10.1038/icb.2011.45

DO - 10.1038/icb.2011.45

M3 - Article

C2 - 21606942

AN - SCOPUS:84857033243

SN - 0818-9641

VL - 90

SP - 159

EP - 167

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

IS - 2

ER -

Interleukin-2 is present in human blood vessels and released in biologically active form by heparanase

Abstract

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

Other files and links

Cite this