Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells

S. K. Mohanty; S. K. Ravula; K. Engisch; A. B. Frazier

doi:10.1109/MMB.2002.1002382

Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells

S. K. Mohanty, S. K. Ravula, K. Engisch, A. B. Frazier

Neuroscience, Cell Biology, and Physiology (NCBP)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Single cell characterization using electrical impedance spectroscopy (EIS) has been conducted on bovine chromaffin cells. Measurements were taken using a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. A population of chromaffin cells were injected into a microreservoir and subsequently manipulated into a microchannel with a cross-sectional area of 7.0×7.0 μm ² . The microchannel was designed with integrated, opposing electroplated palladium electrodes with a cross-sectional area of 7.0×7.0 μm ² . The electrodes are designed to be in contact with the cells during analysis procedures. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. Magnitude and phase were studied for a statistically relevant population of chromaffin cells. The impedance spectrums revealed three distinct populations of chromaffin cells. The data obtained for the chromaffin cells was compared to data obtained for red blood cells, air, and phosphate buffered solution.

Original language	American English
Title of host publication	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578)
Editors	David Beebe, Andre Dittmar
Publisher	IEEE
Pages	485-488
Number of pages	4
ISBN (Print)	0-7803-7480-0
DOIs	https://doi.org/10.1109/MMB.2002.1002382
State	Published - Aug 7 2002
Event	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States Duration: May 2 2002 → May 4 2002

Conference

Conference	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
Country/Territory	United States
City	Madison
Period	5/2/02 → 5/4/02

ASJC Scopus Subject Areas

Biotechnology
Microbiology (medical)

Keywords

MEMS
cell sorting
chromafin cells
electrical impedance spectroscopy
microfluidic

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Neurosciences
Physiological Processes

Access to Document

10.1109/MMB.2002.1002382License: Unspecified

Cite this

Mohanty, S. K., Ravula, S. K., Engisch, K., & Frazier, A. B. (2002). Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells. In D. Beebe, & A. Dittmar (Eds.), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578) (pp. 485-488). Article 1002382 IEEE. https://doi.org/10.1109/MMB.2002.1002382

Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells. / Mohanty, S. K.; Ravula, S. K.; Engisch, K. et al.
2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578). ed. / David Beebe; Andre Dittmar. IEEE, 2002. p. 485-488 1002382.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Mohanty, SK, Ravula, SK, Engisch, K & Frazier, AB 2002, Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells. in D Beebe & A Dittmar (eds), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578)., 1002382, IEEE, pp. 485-488, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 5/2/02. https://doi.org/10.1109/MMB.2002.1002382

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abstract = " Single cell characterization using electrical impedance spectroscopy (EIS) has been conducted on bovine chromaffin cells. Measurements were taken using a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. A population of chromaffin cells were injected into a microreservoir and subsequently manipulated into a microchannel with a cross-sectional area of 7.0×7.0 μm 2 . The microchannel was designed with integrated, opposing electroplated palladium electrodes with a cross-sectional area of 7.0×7.0 μm 2 . The electrodes are designed to be in contact with the cells during analysis procedures. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. Magnitude and phase were studied for a statistically relevant population of chromaffin cells. The impedance spectrums revealed three distinct populations of chromaffin cells. The data obtained for the chromaffin cells was compared to data obtained for red blood cells, air, and phosphate buffered solution.",

keywords = "MEMS, cell sorting, chromafin cells, electrical impedance spectroscopy, microfluidic",

author = "Mohanty, {S. K.} and Ravula, {S. K.} and K. Engisch and Frazier, {A. B.}",

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N2 - Single cell characterization using electrical impedance spectroscopy (EIS) has been conducted on bovine chromaffin cells. Measurements were taken using a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. A population of chromaffin cells were injected into a microreservoir and subsequently manipulated into a microchannel with a cross-sectional area of 7.0×7.0 μm 2 . The microchannel was designed with integrated, opposing electroplated palladium electrodes with a cross-sectional area of 7.0×7.0 μm 2 . The electrodes are designed to be in contact with the cells during analysis procedures. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. Magnitude and phase were studied for a statistically relevant population of chromaffin cells. The impedance spectrums revealed three distinct populations of chromaffin cells. The data obtained for the chromaffin cells was compared to data obtained for red blood cells, air, and phosphate buffered solution.

AB - Single cell characterization using electrical impedance spectroscopy (EIS) has been conducted on bovine chromaffin cells. Measurements were taken using a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. A population of chromaffin cells were injected into a microreservoir and subsequently manipulated into a microchannel with a cross-sectional area of 7.0×7.0 μm 2 . The microchannel was designed with integrated, opposing electroplated palladium electrodes with a cross-sectional area of 7.0×7.0 μm 2 . The electrodes are designed to be in contact with the cells during analysis procedures. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. Magnitude and phase were studied for a statistically relevant population of chromaffin cells. The impedance spectrums revealed three distinct populations of chromaffin cells. The data obtained for the chromaffin cells was compared to data obtained for red blood cells, air, and phosphate buffered solution.

KW - MEMS

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ER -

Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells

Abstract

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