Abstract
The sulphydryl reagent, p -chloromercuribenzenesulfonic acid (PCMB S), irreversible excites the carotid body in vivo. We tested the effects of PCMBS on ionic currents in isolated type I cells, using the whole-cell patch-clamp technique. PCMBS selectively and irreversibly inhibited the Ca 2+ -activated K + current (IK ca ) in a dose-dependent manner (0.01–1 mM). The same concentrations of PCMBS did not affect the Ca 2+ -independent K + current (IK v ), but caused a transient enhancement of Ca 2+ current (I ca ). The inhibition of IK ca by PCMBS is similar to the previously reported effects of hypoxia, and suggests a central role for the channels underlying this current in chemotransduction.
| Original language | English |
|---|---|
| Pages (from-to) | 341-344 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 591 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 25 1992 |
ASJC Scopus Subject Areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Keywords
- Ca-activated K channel
- Carotid body
- Chemoreceptor
- K channel
- p-chloromercuribenzenesulfonic acid
- Type I cell
Disciplines
- Medical Cell Biology
- Medical Neurobiology
- Medical Physiology
- Medical Sciences
- Medicine and Health Sciences
- Neurosciences
- Physiological Processes