Molecular Changes Associated with Replication of Simian Immunodeficiency Virus in Human Cells

Toshiaki Kodama; Dawn P. Wooley; Harry W. Kestler; Muthiah D. Daniel; Ronald C. Desrosiers

Molecular Changes Associated with Replication of Simian Immunodeficiency Virus in Human Cells

Toshiaki Kodama, Dawn P. Wooley, Harry W. Kestler, Muthiah D. Daniel, Ronald C. Desrosiers

Wright State University

Research output: Contribution to journal › Article › peer-review

Abstract

The SIVmac239 infectious clone does not have a premature stop codon in its transmembrane protein (TMP) region and it produces full-length (41 kilodalton, kDa) TMP in macaque peripheral blood lymphocytes (PBL) in vitro and in vivo. However, viruses with truncated forms of TMP (28kDa) are selected during propagation in human cell types; truncated forms arise from point mutations, CAG (glutamine) to TAG (stop), in the viral genome. These results document molecular changes associated with adaptation of SIVmac for growth in human cells.

Original language	American English
Journal	Journal of Medical Primatology
Volume	19
State	Published - Jan 1 1990

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Medical Sciences
Medicine and Health Sciences
Neurosciences
Physiological Processes

Cite this

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title = "Molecular Changes Associated with Replication of Simian Immunodeficiency Virus in Human Cells",

abstract = " The SIVmac239 infectious clone does not have a premature stop codon in its transmembrane protein (TMP) region and it produces full-length (41 kilodalton, kDa) TMP in macaque peripheral blood lymphocytes (PBL) in vitro and in vivo. However, viruses with truncated forms of TMP (28kDa) are selected during propagation in human cell types; truncated forms arise from point mutations, CAG (glutamine) to TAG (stop), in the viral genome. These results document molecular changes associated with adaptation of SIVmac for growth in human cells.",

author = "Toshiaki Kodama and Wooley, {Dawn P.} and Kestler, {Harry W.} and Daniel, {Muthiah D.} and Desrosiers, {Ronald C.}",

year = "1990",

month = jan,

day = "1",

language = "American English",

volume = "19",

journal = "Journal of Medical Primatology",

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TY - JOUR

T1 - Molecular Changes Associated with Replication of Simian Immunodeficiency Virus in Human Cells

AU - Kodama, Toshiaki

AU - Wooley, Dawn P.

AU - Kestler, Harry W.

AU - Daniel, Muthiah D.

AU - Desrosiers, Ronald C.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The SIVmac239 infectious clone does not have a premature stop codon in its transmembrane protein (TMP) region and it produces full-length (41 kilodalton, kDa) TMP in macaque peripheral blood lymphocytes (PBL) in vitro and in vivo. However, viruses with truncated forms of TMP (28kDa) are selected during propagation in human cell types; truncated forms arise from point mutations, CAG (glutamine) to TAG (stop), in the viral genome. These results document molecular changes associated with adaptation of SIVmac for growth in human cells.

AB - The SIVmac239 infectious clone does not have a premature stop codon in its transmembrane protein (TMP) region and it produces full-length (41 kilodalton, kDa) TMP in macaque peripheral blood lymphocytes (PBL) in vitro and in vivo. However, viruses with truncated forms of TMP (28kDa) are selected during propagation in human cell types; truncated forms arise from point mutations, CAG (glutamine) to TAG (stop), in the viral genome. These results document molecular changes associated with adaptation of SIVmac for growth in human cells.

UR - https://corescholar.libraries.wright.edu/ncbp/961

M3 - Article

VL - 19

JO - Journal of Medical Primatology

JF - Journal of Medical Primatology

ER -

Molecular Changes Associated with Replication of Simian Immunodeficiency Virus in Human Cells

Abstract

Disciplines

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