New primers reveal the presence of a duplicate histone H3 in the marine turtle leech Ozobranchus branchiatus

Philip Lavretsky; Triet M. Truong; Audrey E. McGowin; George H. Balazs; Jeffrey L. Peters

doi:10.1007/s12686-011-9581-6

New primers reveal the presence of a duplicate histone H3 in the marine turtle leech Ozobranchus branchiatus

Philip Lavretsky, Triet M. Truong, Audrey E. McGowin, George H. Balazs, Jeffrey L. Peters

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Marine leeches, specific to sea turtles, have been implicated as potential vector organisms in the spread of fibropapillomatosis (FP), a pandemic neoplastic disease with Chelonia mydas having the highest affliction rate. Polymerase chain reaction identified two independent, seemingly functional histone H3 loci for marine turtle leeches Ozobranchus branchiatus collected from C. mydas in Florida and Hawaii. Primers were developed to amplify each product separately. Among the two loci, sequence differentiation (ΦST) ranged from 0.161 to 0.182 with identical amino acid translations among the 22 samples. A maximum parsimony tree of GenBank histone H3 sequences from annelids indicated the gene duplication occurred within the Ozobranchidae family. Geographically separated populations yielded ΦST values of 0.004-0.005 but were phylogenetically distinctive. These novel markers will be useful in identifying ectoparasites in FP research, evaluating other histone variants, and chromatin dynamics regulation studies.

Original language	English
Pages (from-to)	487-490
Number of pages	4
Journal	Conservation Genetics Resources
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/s12686-011-9581-6
State	Published - Jun 2012

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

Keywords

Chelonia mydas
Fibropapillomatosis
Gene duplication
Histone H3
Ozobranchus branchiatus
Primers

Disciplines

Genetics

Access to Document

10.1007/s12686-011-9581-6

Cite this

@article{5d349cf0f21448ccba08447ec1906782,

title = "New primers reveal the presence of a duplicate histone H3 in the marine turtle leech Ozobranchus branchiatus",

abstract = "Marine leeches, specific to sea turtles, have been implicated as potential vector organisms in the spread of fibropapillomatosis (FP), a pandemic neoplastic disease with Chelonia mydas having the highest affliction rate. Polymerase chain reaction identified two independent, seemingly functional histone H3 loci for marine turtle leeches Ozobranchus branchiatus collected from C. mydas in Florida and Hawaii. Primers were developed to amplify each product separately. Among the two loci, sequence differentiation (ΦST) ranged from 0.161 to 0.182 with identical amino acid translations among the 22 samples. A maximum parsimony tree of GenBank histone H3 sequences from annelids indicated the gene duplication occurred within the Ozobranchidae family. Geographically separated populations yielded ΦST values of 0.004-0.005 but were phylogenetically distinctive. These novel markers will be useful in identifying ectoparasites in FP research, evaluating other histone variants, and chromatin dynamics regulation studies.",

keywords = "Chelonia mydas, Fibropapillomatosis, Gene duplication, Histone H3, Ozobranchus branchiatus, Primers",

author = "Philip Lavretsky and Truong, {Triet M.} and McGowin, {Audrey E.} and Balazs, {George H.} and Peters, {Jeffrey L.}",

year = "2012",

month = jun,

doi = "10.1007/s12686-011-9581-6",

language = "English",

volume = "4",

pages = "487--490",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

number = "2",

}

TY - JOUR

T1 - New primers reveal the presence of a duplicate histone H3 in the marine turtle leech Ozobranchus branchiatus

AU - Lavretsky, Philip

AU - Truong, Triet M.

AU - McGowin, Audrey E.

AU - Balazs, George H.

AU - Peters, Jeffrey L.

PY - 2012/6

Y1 - 2012/6

N2 - Marine leeches, specific to sea turtles, have been implicated as potential vector organisms in the spread of fibropapillomatosis (FP), a pandemic neoplastic disease with Chelonia mydas having the highest affliction rate. Polymerase chain reaction identified two independent, seemingly functional histone H3 loci for marine turtle leeches Ozobranchus branchiatus collected from C. mydas in Florida and Hawaii. Primers were developed to amplify each product separately. Among the two loci, sequence differentiation (ΦST) ranged from 0.161 to 0.182 with identical amino acid translations among the 22 samples. A maximum parsimony tree of GenBank histone H3 sequences from annelids indicated the gene duplication occurred within the Ozobranchidae family. Geographically separated populations yielded ΦST values of 0.004-0.005 but were phylogenetically distinctive. These novel markers will be useful in identifying ectoparasites in FP research, evaluating other histone variants, and chromatin dynamics regulation studies.

AB - Marine leeches, specific to sea turtles, have been implicated as potential vector organisms in the spread of fibropapillomatosis (FP), a pandemic neoplastic disease with Chelonia mydas having the highest affliction rate. Polymerase chain reaction identified two independent, seemingly functional histone H3 loci for marine turtle leeches Ozobranchus branchiatus collected from C. mydas in Florida and Hawaii. Primers were developed to amplify each product separately. Among the two loci, sequence differentiation (ΦST) ranged from 0.161 to 0.182 with identical amino acid translations among the 22 samples. A maximum parsimony tree of GenBank histone H3 sequences from annelids indicated the gene duplication occurred within the Ozobranchidae family. Geographically separated populations yielded ΦST values of 0.004-0.005 but were phylogenetically distinctive. These novel markers will be useful in identifying ectoparasites in FP research, evaluating other histone variants, and chromatin dynamics regulation studies.

KW - Chelonia mydas

KW - Fibropapillomatosis

KW - Gene duplication

KW - Histone H3

KW - Ozobranchus branchiatus

KW - Primers

UR - http://www.scopus.com/inward/record.url?scp=84892852616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892852616&partnerID=8YFLogxK

U2 - 10.1007/s12686-011-9581-6

DO - 10.1007/s12686-011-9581-6

M3 - Article

AN - SCOPUS:84892852616

SN - 1877-7252

VL - 4

SP - 487

EP - 490

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

New primers reveal the presence of a duplicate histone H3 in the marine turtle leech Ozobranchus branchiatus

Abstract

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

Other files and links

Cite this