Oxidative Stress Decreases pHi and Na+/H+ Exchange and Increases Excitability of Solitary Complex Neurons from Rat Brain Slices

Putative chemoreceptors in the solitary complex (SC) are sensitive to hypercapnia and oxidative stress. We tested the hypothesis that oxidative stress stimulates SC neurons by a mechanism independent of intracellular pH (pH _i ). pH _i was measured by using ratiometric fluorescence imaging microscopy, utilizing either the pH-sensitive fluorescent dye BCECF or, during whole cell recordings, pyranine in SC neurons in brain stem slices from rat pups. Oxidative stress decreased pH _i in 270 of 436 (62%) SC neurons tested. Chloramine-T (CT), N -chlorosuccinimide (NCS), dihydroxyfumaric acid, and H ₂ O ₂ decreased pH _i by 0.19 ± 0.007, 0.20 ± 0.015, 0.15 ± 0.013, and 0.08 ± 0.002 pH unit, respectively. Hypercapnia decreased pH _i by 0.26 ± 0.006 pH unit ( n = 95). The combination of hypercapnia and CT or NCS had an additive effect on pH _i , causing a 0.42 ± 0.03 ( n = 21) pH unit acidification. CT slowed pH _i recovery mediated by Na ⁺ /H ⁺ exchange (NHE) from NH ₄ Cl-induced acidification by 53% ( n = 20) in -buffered medium and by 58% ( n = 10) in HEPES-buffered medium. CT increased firing rate in 14 of 16 SC neurons, and there was no difference in the firing rate response to CT with or without a corresponding change in pH _i . These results indicate that oxidative stress 1 ) decreases pH _i in some SC neurons, 2 ) together with hypercapnia has an additive effect on pH _i , 3 ) partially inhibits NHE, and 4 ) directly affects excitability of CO ₂ /H ⁺ -chemosensitive SC neurons independently of pH _i changes. These findings suggest that oxidative stress acidifies SC neurons in part by inhibiting NHE, and this acidification may contribute ultimately to respiratory control dysfunction.