Regulation of Potassium Transport in Human Lens Epithelial Cells

Peter K. Lauf; Ronald E. Warwar; Thomas L. Brown; Norma C. Adragna

doi:10.1016/j.exer.2005.05.002

Regulation of Potassium Transport in Human Lens Epithelial Cells

Peter K. Lauf, Ronald E. Warwar, Thomas L. Brown, Norma C. Adragna

Research output: Contribution to journal › Article › peer-review

Abstract

The major K influx pathways and their response to thiol modification by N-ethylmaleimide (NEM) and protein kinase and phosphatase inhibitors were characterized in human lens epithelial B3 (HLE-B3) cells with Rb as K congener. Ouabain (0.1mM) and bumetanide (5μM) discriminated between the Na/K pump (∼35% of total Rb influx) and Na–K–2Cl cotransport (NKCC) (∼50%). Cl-replacement with nitrate or sulfamate revealed 100μM, activated the Na/K pump and abolished NKCC but did not affect KCC. The data suggest at least partial inverse regulation of KCC and NKCC in HLE-B3 cells by signaling cascades involving serine, threonine and tyrosine phosphorylation/dephosphorylation equilibria.

Original language	American English
Journal	Experimental Eye Research
Volume	82
DOIs	https://doi.org/10.1016/j.exer.2005.05.002
State	Published - Jan 1 2006

Keywords

human lens epithelial cells
cation–chloride cotransporters
Na/K pump
K–Cl cotransport (KCC)
Na–K–2Cl cotransport (NKCC)
N-ethylmaleimide
protein kinases
protein phosphatases
loop diuretics
cataract

Disciplines

Medical Cell Biology
Medical Neurobiology
Medical Physiology
Medical Sciences
Medicine and Health Sciences
Neurosciences
Physiological Processes

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10.1016/j.exer.2005.05.002License: CC BY

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title = "Regulation of Potassium Transport in Human Lens Epithelial Cells",

abstract = " The major K influx pathways and their response to thiol modification by N-ethylmaleimide (NEM) and protein kinase and phosphatase inhibitors were characterized in human lens epithelial B3 (HLE-B3) cells with Rb as K congener. Ouabain (0.1mM) and bumetanide (5μM) discriminated between the Na/K pump (∼35% of total Rb influx) and Na–K–2Cl cotransport (NKCC) (∼50%). Cl-replacement with nitrate or sulfamate revealed 100μM, activated the Na/K pump and abolished NKCC but did not affect KCC. The data suggest at least partial inverse regulation of KCC and NKCC in HLE-B3 cells by signaling cascades involving serine, threonine and tyrosine phosphorylation/dephosphorylation equilibria.",

keywords = "human lens epithelial cells, cation–chloride cotransporters, Na/K pump, K–Cl cotransport (KCC), Na–K–2Cl cotransport (NKCC), N-ethylmaleimide, protein kinases, protein phosphatases, loop diuretics, cataract",

author = "Lauf, {Peter K.} and Warwar, {Ronald E.} and Brown, {Thomas L.} and Adragna, {Norma C.}",

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T1 - Regulation of Potassium Transport in Human Lens Epithelial Cells

AU - Lauf, Peter K.

AU - Warwar, Ronald E.

AU - Brown, Thomas L.

AU - Adragna, Norma C.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The major K influx pathways and their response to thiol modification by N-ethylmaleimide (NEM) and protein kinase and phosphatase inhibitors were characterized in human lens epithelial B3 (HLE-B3) cells with Rb as K congener. Ouabain (0.1mM) and bumetanide (5μM) discriminated between the Na/K pump (∼35% of total Rb influx) and Na–K–2Cl cotransport (NKCC) (∼50%). Cl-replacement with nitrate or sulfamate revealed 100μM, activated the Na/K pump and abolished NKCC but did not affect KCC. The data suggest at least partial inverse regulation of KCC and NKCC in HLE-B3 cells by signaling cascades involving serine, threonine and tyrosine phosphorylation/dephosphorylation equilibria.

AB - The major K influx pathways and their response to thiol modification by N-ethylmaleimide (NEM) and protein kinase and phosphatase inhibitors were characterized in human lens epithelial B3 (HLE-B3) cells with Rb as K congener. Ouabain (0.1mM) and bumetanide (5μM) discriminated between the Na/K pump (∼35% of total Rb influx) and Na–K–2Cl cotransport (NKCC) (∼50%). Cl-replacement with nitrate or sulfamate revealed 100μM, activated the Na/K pump and abolished NKCC but did not affect KCC. The data suggest at least partial inverse regulation of KCC and NKCC in HLE-B3 cells by signaling cascades involving serine, threonine and tyrosine phosphorylation/dephosphorylation equilibria.

KW - human lens epithelial cells

KW - cation–chloride cotransporters

KW - Na/K pump

KW - K–Cl cotransport (KCC)

KW - Na–K–2Cl cotransport (NKCC)

KW - N-ethylmaleimide

KW - protein kinases

KW - protein phosphatases

KW - loop diuretics

KW - cataract

UR - https://corescholar.libraries.wright.edu/ncbp/50

U2 - 10.1016/j.exer.2005.05.002

DO - 10.1016/j.exer.2005.05.002

M3 - Article

C2 - 16002066

VL - 82

JO - Experimental Eye Research

JF - Experimental Eye Research

ER -

Regulation of Potassium Transport in Human Lens Epithelial Cells

Abstract

Keywords

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